FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18850723
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG54950.020.190973
AAAGTGC310900.019.9663264
CCAAAGT370950.018.5515252
AAGTGCT353600.018.1908155
AGTAGCT207850.017.9396275
CCGAGTA62700.017.9193122
GCTGGGA586100.017.8907939
CCCAAAG450300.017.0344411
CAAAGTG390150.016.9725783
TAGCTGG236250.016.8260697
CGTCGAG26000.016.4802441
CTACTCG52400.016.2152252
GAGTAGC143500.016.0504994
AGTGCTG419400.015.4563416
GCTACTC140000.015.2027371
GTAGCTG244050.014.9643536
GTCGAGA41100.014.0145511
TACTCGG61700.013.8848913
CTACTTG139450.013.394672
GGAGGCT434750.013.3600299
Produced by FastQC (version 0.11.5)