FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18864273
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG52400.021.596263
AAAGTGC312950.021.257454
CCAAAGT371900.019.3737852
AAGTGCT353350.018.8391285
CCGAGTA58200.018.4973352
CTACTCG56800.018.4577472
AGTAGCT202650.018.3994795
CAAAGTG380700.018.2783183
GCTGGGA587700.018.2514049
CCCAAAG444550.018.2304731
TAGCTGG235450.017.3838397
GAGTAGC140450.016.7055534
TACTCGG65450.016.216283
GTAGCTG242850.015.9017236
AGTGCTG428000.015.4206846
GCTACTC159450.015.2348361
TTGGGAG345500.013.4329996
GGAGGCT440100.013.2549559
GCTACTT145750.013.1402471
CCCGAGT96650.012.8219481
Produced by FastQC (version 0.11.5)