FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15927741
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC219800.016.5270354
CCAAAGT268300.015.4357692
AAGTGCT257950.014.4390525
CAAAGTG279400.013.8867743
GCTGGGA475350.013.8271639
CCCAAAG344850.013.7523751
AGTAGCT140850.013.3471455
CTACTCG39600.013.2065042
TAGCTGG174050.013.0753597
CTGTCTA117000.013.027804145
CGAGTAG39450.012.7194053
CCTATAT57650.012.3824761
GCTATAT58250.012.01225951
AGTGCTG344750.011.2135726
CGTCGAG27100.010.9538151
GAGTAGC103150.010.8254854
GCTACTC104800.010.5882411
CCGAGTA46250.010.5435942
TACTCGG49600.010.1165433
GTAGCTG190950.010.1042456
Produced by FastQC (version 0.11.5)