FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16287239
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC231550.018.4868074
CCAAAGT278250.016.5028722
AAGTGCT265100.016.3874995
CAAAGTG291500.015.9227463
CCCAAAG349250.015.2786961
GCTGGGA489150.014.2908549
CGAGTAG44450.014.1667723
CCGAGTA52600.013.1822742
AGTAGCT148200.013.17694955
AGTGCTG349150.012.7667756
TAGCTGG177850.012.3721327
GAGTAGC107350.012.0614744
CCTGTCG91600.011.073511145
CTACTCG40050.010.776492
CTGTCTA125250.010.730475145
GCTAATT109400.010.3503261
GTGCTGG479400.010.2866227
CCTATAT62700.010.2712961
TGCTGGG545650.010.178798
GTAGCTG200300.09.9967356
Produced by FastQC (version 0.11.5)