FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16361564
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC220850.018.7917064
CCAAAGT268550.016.8280772
AAGTGCT259050.015.8839985
CAAAGTG289400.015.6378223
CTGTCTA135300.015.589691145
CCCAAAG348750.015.5856871
GCTGGGA478150.015.4051889
CGAGTAG41800.014.0629283
AGTAGCT145650.013.6149125
CTACTCG44700.013.4694662
TAGCTGG175250.013.4578617
GCTACTC105800.013.3964321
AGTGCTG338450.012.8271626
CCGAGTA50800.011.4337612
CGCGATA13202.045621E-611.346343145
GAGTAGC106000.011.0241224
GCTAATT109650.010.9225211
TACTCGG52750.010.8751813
CTCGACG82600.010.728201145
GCTACTT112050.010.6885721
Produced by FastQC (version 0.11.5)