FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16361564
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC227700.017.8575084
CCAAAGT272050.017.1071622
AAGTGCT258350.015.9036025
CCCAAAG352000.015.4585531
CAAAGTG293150.015.2476233
GCTGGGA475250.014.622549
CGAGTAG42850.014.5477083
AGTAGCT145500.014.2166045
CTGTCTA126400.014.076408145
TAGCTGG173700.013.7032847
CTACTCG40650.013.5923772
CCTGTCG94800.012.246143145
AGTGCTG343450.012.1897826
GCTACTC110000.011.78714751
CCGAGTA52400.011.7611382
GCTAATA60000.011.5724441
GAGTAGC107050.011.2495264
ATCTACG118900.011.143646145
GCTATAT57750.011.0418231
GTAGCTG198950.010.8243456
Produced by FastQC (version 0.11.5)