FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14942690
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC202250.017.8279024
CCAAAGT244600.016.8553962
CCCAAAG307150.015.7774781
CAAAGTG255150.015.5476043
TAGCTGG163050.015.4220037
AAGTGCT238450.015.2996275
GCTGGGA422200.014.9820359
AGTAGCT139150.014.9161035
CGAGTAG41750.014.4222633
CTGTCTA121800.013.459621145
CGCGTAA10502.093253E-613.010968145
CTACTCG41300.012.521182
GAGTAGC102450.012.5151944
AGTGCTG306800.012.3990936
CCGAGTA49400.012.3322952
GTAGCTG182350.011.8485456
CGTACTA38300.011.349467145
TACTCGG50600.011.3398023
GCTACTC100300.011.0899791
CCTATAT60850.010.9445851
Produced by FastQC (version 0.11.5)