FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14942690
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC201450.019.8539474
CTGTCTA128350.018.242638145
AAGTGCT231200.017.8203745
CCAAAGT244100.017.5154082
CAAAGTG255750.016.6624093
CCCAAAG304600.015.9921571
GCTGGGA426350.015.1614729
AGTGCTG305950.014.3699816
TAGCTGG158350.013.5917837
CGCGATA11855.084803E-813.378495145
AGTAGCT137350.013.2975835
CGAGTAG39250.012.4441823
GCTACTC100300.012.42396451
TCGACGG27850.012.2606945145
CTACTCG42850.012.2245252
CCGAGTA44900.012.1393532
GAGTAGC100750.011.8040184
GCTAATT103750.011.2601541
CCTGTCG90700.011.159714145
GTGCTGG422650.011.1561477
Produced by FastQC (version 0.11.5)