FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14746814
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC197800.018.189834
CCAAAGT242700.016.8090782
AAGTGCT229550.015.92075355
CCCAAAG300350.015.42688751
CTGTCTA123300.015.330071145
CAAAGTG252700.015.0508233
GCTGGGA426450.014.8315449
CGTCGAG32050.014.3685691
AGTAGCT132650.013.98895555
TAGCTGG161350.013.6080447
AGTGCTG297150.013.22843556
CTACTCG39750.013.0069952
CGAGTAG39450.012.5673083
GCTACTC99650.012.1575761
GTCGAGA37150.011.820182
GCTATAT55350.011.6480161
GAGTAGC98150.011.5457164
TACTCGG45450.011.064093
CCTATAT58000.010.993671
GTAGCTG178300.010.88404756
Produced by FastQC (version 0.11.5)