FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14746814
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC196250.018.0406444
CCAAAGT235250.016.916432
CTGTCTA128150.016.789055145
AAGTGCT225350.016.2140925
CCCAAAG303400.015.5718571
CAAAGTG250700.015.3933233
GCTGGGA412150.014.9825869
AGTAGCT131300.014.0219755
GCTACTC97400.013.8173511
TAGCTGG157100.012.98183257
TCGACGG28300.012.958865145
CTACTCG41400.012.6570762
AGTGCTG298800.012.6077886
CCTATAT57100.012.5289711
CGAGTAG39100.012.4957573
TACTCGG46850.012.2423733
CTCGACG101950.011.511084145
CCGAGTA46650.011.0808532
GCTAATT102850.010.9502231
GGAGGCT322600.010.9098219
Produced by FastQC (version 0.11.5)