FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14443318
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG24250.024.5421621
ACGTCGA18450.020.492881
CGAGTAG37250.018.0446153
AAAGTGC220550.016.8255754
GTCGAGA35300.016.4630012
CTACTCG36200.016.4405362
CCGAGTA44850.016.3920212
AGTAGCT143400.015.8686995
CCAAAGT266100.015.68222
GCTGGGA434200.014.7872249
AAGTGCT253150.014.7143425
TAGCTGG164150.014.3314137
CCCAAAG330350.014.2853621
CAAAGTG278000.014.2552323
TACTCGG45350.013.58651353
GCTACTC98650.013.4853481
GAGTAGC96300.013.2326324
GTAGCTG178050.012.9380876
TCGAGAC55000.011.8392143
AGTGCTG322000.011.7205796
Produced by FastQC (version 0.11.5)