FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14443318
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC218150.016.5291654
CTACTCG34950.015.8393342
CCAAAGT262150.015.476962
CGAGTAG36450.014.9816573
CCCAAAG326550.014.7801621
CAAAGTG274250.014.4998833
AGTAGCT141250.014.2756835
AAGTGCT252950.014.0611845
GCTGGGA434900.013.9750969
GCTACTC95600.013.5754521
TAGCTGG167400.013.3846947
CCGAGTA43900.013.2486182
TACTCGG43800.012.9471363
GAGTAGC96550.012.7631914
GTAGCTG175000.011.5624846
AGTGCTG314750.011.478246
GCTACTT105100.011.2136511
GGAGGCT326100.010.9292779
GCTATAT56300.010.4667381
GCTAATT116100.010.3324751
Produced by FastQC (version 0.11.5)