FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14751396
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC225600.018.0765424
CGAGTAG35500.017.7648643
CCAAAGT273200.016.9329722
AAGTGCT260400.015.4728975
AGTAGCT139550.015.4655585
TAGCTGG161500.015.4037717
CCGAGTA43900.015.3276872
CAAAGTG285400.015.2961383
CCCAAAG338700.015.1159481
CTACTCG36000.014.991952
GCTGGGA445850.014.9479169
GAGTAGC96300.014.552424
TACTCGG46150.013.5134423
GCTACTC102600.013.1244651
AGTGCTG324900.012.5090126
GTAGCTG176600.012.3796586
GCTATAT59400.012.0431871
GCTATAC40450.011.2699651
GCTAATT118600.010.8216661
GCTACTT105000.010.620271
Produced by FastQC (version 0.11.5)