FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14441752
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA21050.042.9384841
CGTCGAG30800.029.5663682
GTCGAGA40300.022.944273
AAAGTGC217150.018.387534
CCAAAGT270400.016.68342
CGAGTAG38800.016.6096883
AAGTGCT253050.016.2221035
AGTAGCT144650.015.4969945
CCGAGTA44150.015.3902762
CAAAGTG281500.015.3536683
CCCAAAG333200.015.3296291
TCGAGAC62500.015.0187284
GCTGGGA439800.014.8925579
TAGCTGG163900.014.745287
CTACTCG36700.014.3153052
GAGTAGC99700.013.5603384
AGTGCTG315950.013.2142436
CGAGACT73750.012.7279565
GTAGCTG179750.012.5098046
GCTATAT53050.011.754761
Produced by FastQC (version 0.11.5)