FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14441752
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC224300.018.9577484
CCAAAGT268250.017.6581062
CCCAAAG330500.017.054711
AAGTGCT261400.016.3754435
CAAAGTG280650.016.1511783
CGAGTAG35900.016.0023733
GCTGGGA443300.015.6787159
AGTAGCT141350.014.9186015
CTACTCG37100.014.7318682
TAGCTGG164750.014.4164147
AGTGCTG321950.013.861496
CCGAGTA42700.013.4562212
GAGTAGC98950.013.3097344
GCTACTC96600.012.5553481
TACTCGG43450.012.2543073
GTAGCTG177700.011.8274956
GTGCTGG413950.011.221447
TGCTGGG474950.010.8720548
CCTATAT60000.010.8665451
TTGGGAG249200.010.767376
Produced by FastQC (version 0.11.5)