FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14751396
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG26550.025.865311
ACGTCGA17700.020.5866761
AAAGTGC230750.018.4305524
GTCGAGA38150.017.9980052
CCAAAGT277050.016.614992
AAGTGCT263900.016.2217485
CCCAAAG341100.016.1266481
CAAAGTG288150.015.6588583
GCTGGGA451500.014.8198699
AGTAGCT145400.014.5043375
CTACTCG35850.014.0713642
TAGCTGG169150.013.9592167
CGAGTAG37400.013.86286453
TCGAGAC60350.013.1187983
GCTACTC97500.012.9367161
GAGTAGC102800.012.6768464
AGTGCTG329450.012.6753916
GCTATAT59550.012.0025791
TACTCGG44000.011.7834353
GTAGCTG183800.011.6648876
Produced by FastQC (version 0.11.5)