FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12463682
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG27600.033.450721
ACGTCGA17700.028.4511531
GTCGAGA37900.024.5444372
CGAGACT64200.018.3026891
TCGAGAC52900.017.848843
AAAGTGC181200.015.8641044
CCAAAGT219500.014.179672
CCCAAAG273450.013.9398861
AAGTGCT211750.013.7076135
CAAAGTG224900.013.3724493
GCTGGGA377400.012.93541059
AGTAGCT108350.012.9103565
CTACTCG29950.012.8441832
GCTATAT48350.012.7299541
GACTTGT112450.012.6882774
GCTAATT88300.012.5151781
CGAGTAG30200.012.0428243
CCTATAT47650.012.0362611
TAGCTGG128650.011.9598957
CCGAGTA35900.011.2998892
Produced by FastQC (version 0.11.5)