FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12463682
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC183200.017.9786194
CCAAAGT217550.016.7620282
AAGTGCT207350.015.9860865
CAAAGTG229850.015.5456683
CCCAAAG273200.015.0298131
GCTGGGA373500.014.0432669
CTACTCG29250.013.6394512
AGTAGCT109400.013.1040145
GCTACTC74750.012.9361351
GCTAATT85700.012.9185241
CCTATAT46550.012.6443421
AGTGCTG275850.012.52343756
TAGCTGG125900.012.4427467
CGAGTAG31450.011.5615393
GCTAATA47250.011.2706361
GAGTAGC80050.011.26914
CCGAGTA36650.010.8855112
TACTCGG36100.010.8471043
GTGCTGG367200.010.1321817
CTATATG47350.010.051642
Produced by FastQC (version 0.11.5)