FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12416801
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA21950.050.071771
CGTCGAG30250.036.0929262
GTCGAGA44200.024.7016073
TCGAGAC56050.020.2285984
AAAGTGC185300.017.8276024
CCAAAGT220550.016.5013392
CGAGACT73750.015.6620921
AAGTGCT212900.015.4179035
CCCAAAG273950.015.2045471
CAAAGTG238800.014.5368483
CTACTCG28150.013.4257392
GCTGGGA382750.013.0927599
AGTGCTG276000.012.9326496
AGTAGCT109450.012.9170625
GCTAATT87900.012.7426261
CCTAATA48700.012.3622441
TAGCTGG126100.012.2660167
CGAGTAG30250.012.0309753
GACTTGT118800.011.4880968
CCTATAT50300.011.2731391
Produced by FastQC (version 0.11.5)