FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12416801
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC170950.017.7666574
CCAAAGT215000.016.6708032
CAAAGTG220350.014.8964113
CCCAAAG270050.014.8355761
AAGTGCT206750.014.5557385
GCTGGGA365750.013.6057619
GCTAATT84150.012.3185651
GCTACTC81000.011.9329161
AGTAGCT105200.011.8417425
TAGCTGG125500.011.8229127
GCTATAT47000.011.7728541
AGTGCTG273200.011.2972966
CTACTCG29500.011.1532362
GCTATTA52050.010.6306281
GCTATAA53600.010.58455851
CCTATAC34500.010.5569091
CGAGTAG27650.010.3779263
CCTAATA47650.010.2893431
GCTATAG41850.010.0417241
CCTATAT46800.010.0272411
Produced by FastQC (version 0.11.5)