FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12301436
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA21500.047.515271
CGTCGAG32300.032.4940642
GTCGAGA43550.024.260843
TCGAGAC59750.017.8001124
AAAGTGC176900.015.9797724
CGAGACT74750.015.8195561
CCAAAGT215950.015.0665912
CCCAAAG274850.014.4091871
CTACTCG31100.014.3990832
CGAGTAG30050.014.203733
TAGCTGG127550.013.5500957
CAAAGTG227700.013.3980173
AAGTGCT214400.013.28276355
GCTACTC79450.013.0342671
GCTGGGA370800.012.4169689
AGTAGCT113800.012.2972095
TACTCGG37250.012.0218353
GACTTGT121350.011.9356923
CCTATAT47000.011.9099771
GCTAATT85900.011.6482761
Produced by FastQC (version 0.11.5)