FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20082711
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA24150.026.7987251
CGTCGAG38400.024.6465451
AAAGTGC306900.018.5689224
GTCGAGA52300.018.2275962
CTACTCG49500.016.868892
CCAAAGT372750.016.7076682
AAGTGCT354000.016.3537585
CCCAAAG455650.015.944741
CAAAGTG387450.015.5705593
GCTGGGA610650.015.1883459
GCTACTC138150.014.5074121
AGTAGCT197150.013.8352425
AGTGCTG443300.013.5145926
CGAGTAG51600.013.4850033
TAGCTGG227300.013.3156537
CTGTCTA143950.012.84523145
TCGAGAC82000.012.8133843
TACTCGG59250.012.68341353
GAGTAGC139100.012.4558084
GACTTGT172000.012.0960483
Produced by FastQC (version 0.11.5)