FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20306802
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG209700.10326589090689908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA147700.018.254524145
AAAGTGC304700.018.068264
CCAAAGT363350.017.0260122
CTACTCG52200.016.663872
AAGTGCT356700.015.90368755
CCCAAAG463000.015.6908041
CAAAGTG383200.015.3828213
GCTGGGA615900.015.06784259
TACTCGG62600.014.6749843
CGAGTAG50200.014.2794533
GCTACTC139700.014.2460771
AGTAGCT198950.014.0645385
TAGCTGG228750.013.9995467
AGTGCTG437100.013.3299556
CCGAGTA59050.013.3166472
GAGTAGC137250.011.9677364
GCTACTT147450.011.8927261
GGAGGCT462000.011.7388799
GTAGCTG244650.011.7229266
GCTAATT162050.011.2506581
Produced by FastQC (version 0.11.5)