FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19707023
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC297600.018.1059384
CCAAAGT355650.017.06142
CGAGTAG47300.016.901663
CTACTCG51800.016.3701732
CCCAAAG441350.015.7811911
AAGTGCT344050.015.6827895
CAAAGTG370400.015.6526453
GCTACTC136350.015.3449871
CTGTCTA141100.015.241387145
GCTGGGA589800.014.777079
AGTAGCT190350.014.3921775
TAGCTGG228000.013.9402817
GAGTAGC129650.013.56751254
CCGAGTA58450.013.5563742
TACTCGG62150.013.422483
AGTGCTG427800.012.5653256
GTAGCTG238450.011.37371356
GGAGGCT450150.011.3108669
GCTAATT158800.011.2933911
GTGCTGG550950.010.4395917
Produced by FastQC (version 0.11.5)