FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19989202
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA31650.050.8403781
CGTCGAG44950.036.2369232
GTCGAGA59300.026.8808273
TCGAGAC89900.018.0408954
AAAGTGC305650.016.4199854
CGAGTAG50650.016.3542023
CCAAAGT365850.015.620872
AGTAGCT196950.015.1621955
CGAGACT111300.014.6346215
AAGTGCT353600.014.5082825
GCTGGGA608000.014.47139
CTACTCG52000.014.3233812
CCCAAAG459250.014.0908461
TAGCTGG227950.014.0774967
CCGAGTA60500.014.0368482
CAAAGTG387550.013.9378523
GCTACTC139300.013.1514951
CTGTCTA145650.013.074258145
GAGTAGC138400.012.82525354
GTAGCTG244100.012.5473876
Produced by FastQC (version 0.11.5)