FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20082711
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG202040.10060394734555508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC297750.018.0207144
CTGTCTA148300.017.685236145
CCAAAGT364000.017.1612912
CGAGTAG49400.016.599893
CTACTCG53350.015.7595082
AAGTGCT350300.015.5380795
CCCAAAG458700.015.4067471
CAAAGTG378350.015.3003523
GCTGGGA601850.014.8233819
CCGAGTA58100.014.5906752
AGTAGCT193700.014.2115455
TAGCTGG225250.014.0756987
GCTACTC138650.014.0332061
TACTCGG64400.012.9492773
GAGTAGC136600.012.7711334
AGTGCTG444600.012.5251566
GCTAATT164000.011.6521921
GTAGCTG247000.011.314866
GCTACTT145450.011.1316861
GGAGGCT454900.011.1281859
Produced by FastQC (version 0.11.5)