FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20306802
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG40600.026.5989251
ACGTCGA26500.024.188021
GTCGAGA55250.019.2878042
CGAGTAG50850.018.216823
AAAGTGC310450.017.3195444
CTACTCG52200.016.2784462
CCAAAGT372800.016.1047882
CCGAGTA61350.015.6670842
CCCAAAG456250.015.4232841
AAGTGCT359000.015.2294765
AGTAGCT192150.015.1873975
GCTGGGA611750.014.6180719
CAAAGTG389150.014.5507493
TACTCGG62800.014.41768553
TAGCTGG233500.013.95908457
TCGAGAC83750.013.8881223
GCTACTC141650.013.67371751
GAGTAGC138800.013.498134
AGTGCTG446100.012.396446
CTGTCTA146600.012.380466145
Produced by FastQC (version 0.11.5)