FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19989202
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC296800.017.6029054
CTACTCG50400.016.83732
CTGTCTA146200.016.777857145
CCAAAGT359300.016.552062
CCCAAAG449400.015.725121
AAGTGCT345700.015.4762125
CAAAGTG375750.015.1621073
GCTGGGA602600.015.0357869
GCTACTC141050.014.4979831
TACTCGG62000.014.1368783
CGAGTAG48050.013.6084643
AGTGCTG442200.013.0281586
TAGCTGG230900.012.6870647
AGTAGCT195200.012.5458635
GGAGGCT453250.011.6839889
CTACTTG131700.011.1439672
TTGGGAG354650.011.0257426
GAGTAGC133650.010.9830214
GCTAATT157150.010.932411
CCGAGTA58650.010.9109592
Produced by FastQC (version 0.11.5)