FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18018810
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC278300.018.1914064
CCAAAGT331950.017.1620712
AAGTGCT312450.016.2502175
CTACTCG45000.016.1517412
CCCAAAG409950.015.9212641
CAAAGTG346550.015.7835633
GCTGGGA528200.015.7174639
CGAGTAG44800.015.1457893
AGTAGCT177000.014.7537965
TAGCTGG201100.014.6431077
GCTACTC127900.014.2866411
GAGTAGC122500.013.4555974
TACTCGG53500.013.3299133
CCGAGTA55150.013.1791182
AGTGCTG392550.012.7604836
GTAGCTG214050.012.0730616
CCTATAT71500.011.8100181
CTACTTG125100.011.3986252
TTGGGAG324200.011.3903736
GCTAATT150500.011.22147751
Produced by FastQC (version 0.11.5)