FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17429192
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG33500.034.951321
ACGTCGA20300.027.6446231
GTCGAGA48300.024.9488532
AAAGTGC276750.017.7168544
TCGAGAC78500.016.6738873
CCAAAGT334200.015.6869292
AAGTGCT311850.015.6338315
GCTGGGA523600.015.3557979
CAAAGTG345200.015.2471113
CCCAAAG401650.014.88622951
CGAGTAG45350.014.6601593
TAGCTGG202300.014.2065777
AGTAGCT182300.013.8657915
CTACTCG45850.013.5941362
CGAGACT92300.013.5055354
CCGAGTA54350.013.1246022
TACTCGG52300.013.1092623
AGTGCTG385700.013.053396
GCTACTC120250.012.5024841
GTAGCTG215100.012.3309596
Produced by FastQC (version 0.11.5)