FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17279747
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA27500.051.6359441
CGTCGAG39800.035.6714942
GTCGAGA53750.026.6711883
AAAGTGC268100.017.5913644
TCGAGAC85250.016.4911484
CCAAAGT321600.016.280072
AAGTGCT313600.015.414475
CGAGTAG42300.015.0625313
GCTGGGA516900.015.0594479
CGAGACT95550.014.9308935
CTACTCG39550.014.8841122
CCCAAAG396050.014.8836851
CAAAGTG334700.014.6289843
CCGAGTA50750.013.23689652
AGTGCTG381200.013.0261336
TACTCGG49050.012.7073213
AGTAGCT179250.012.6725475
TAGCTGG204250.012.40997
GCTACTC118050.012.3220851
GAGTAGC121200.011.599594
Produced by FastQC (version 0.11.5)