FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17573635
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA27200.052.482481
CGTCGAG41200.035.1309172
GTCGAGA57450.024.8322683
AAAGTGC277950.016.3695934
TCGAGAC88550.016.3454514
CCAAAGT329600.015.44331552
CGAGACT99350.014.5685925
AAGTGCT310700.014.5549635
GCTGGGA528450.014.481099
AGTAGCT175900.014.4490545
CAAAGTG341850.014.3834113
CGAGTAG46900.014.1754733
CCCAAAG412950.014.1128441
CTACTCG43950.014.0239892
TAGCTGG204850.013.6918057
GAGTAGC118600.013.3134114
CCGAGTA53450.013.2157812
AGTGCTG385450.012.2534386
GTAGCTG214250.012.0567336
GCTACTC117000.011.7272281
Produced by FastQC (version 0.11.5)