FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18018810
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG36050.033.8269961
ACGTCGA23550.027.9504451
GTCGAGA52450.023.5113472
CGAGTAG44550.017.5725083
AAAGTGC285750.017.16524
CCAAAGT340250.015.7189012
AGTAGCT181700.015.2894295
GCTGGGA531800.015.0849939
AAGTGCT323250.015.0238525
TCGAGAC83800.014.8809793
CCGAGTA52700.014.8549372
CCCAAAG426000.014.6870371
CAAAGTG351250.014.6743753
CTACTCG48300.014.3435242
TAGCTGG207450.014.192557
GCTACTC127500.014.1292531
GAGTAGC121500.013.2856074
AGTGCTG391900.012.8870666
GTAGCTG219600.012.6191316
CGAGACT97950.012.5897574
Produced by FastQC (version 0.11.5)