FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17573635
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC264900.017.1973654
CCAAAGT319700.015.6309412
CGAGTAG43400.015.3135533
CTACTCG47950.015.1562342
AAGTGCT303100.015.1000385
GCTGGGA523900.014.7677149
AGTAGCT172350.014.7440655
CCCAAAG399550.014.4473431
TAGCTGG205800.014.200997
CAAAGTG338050.014.1921033
GCTACTC123700.014.1561091
CCGAGTA50100.012.8480092
GAGTAGC117150.012.5879574
GTAGCTG212950.012.4877836
TACTCGG57800.012.3368383
AGTGCTG375900.012.2512696
GGAGGCT385350.011.1440949
TTGGGAG303950.010.6173356
GCTACTT122700.010.4356681
GTGCTGG482050.010.2147817
Produced by FastQC (version 0.11.5)