FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21388249
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA26350.030.9834061
CGTCGAG40450.027.8389741
GTCGAGA57500.020.19222
AAAGTGC311600.016.891794
CCAAAGT379250.016.0308152
AAGTGCT369650.014.6765875
CAAAGTG401550.014.6675433
CCCAAAG481300.014.6376031
CGAGTAG50400.014.3806413
TCGAGAC86850.014.3408543
CTACTCG53550.014.1916122
GCTGGGA644500.014.0184979
GCTACTC142500.013.5821841
AGTAGCT195350.013.5796745
TAGCTGG233650.012.8292777
TACTCGG65100.012.4304943
CCGAGTA64100.012.07541752
AGTGCTG472100.012.043146
GCTATAT78500.011.9243241
GAGTAGC136250.011.1554944
Produced by FastQC (version 0.11.5)