FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20772632
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC308400.017.2887124
CCAAAGT371500.015.9179062
AAGTGCT353700.015.3128355
CGAGTAG47450.015.2480483
CTACTCG50850.014.9164492
CAAAGTG387200.014.4952153
CCCAAAG474300.013.8602851
GCTACTC141850.013.764641
GCTGGGA632100.013.7498729
TAGCTGG221150.013.7254727
AGTAGCT194450.013.5475145
TACTCGG59500.013.2225223
CCGAGTA59250.012.3275752
AGTGCTG451500.012.3082966
GAGTAGC132500.011.8229064
GCTATAT78800.011.5868811
GCTAATT153700.011.5153211
GTAGCTG245150.010.7181266
GCTACTT140500.010.2977021
GTGCTGG599900.010.1079357
Produced by FastQC (version 0.11.5)