FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15405534
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC241350.019.0199074
CTACTCG41750.018.834262
CCAAAGT297350.017.3676742
CCCAAAG360600.016.7433591
AAGTGCT276650.016.541795
CAAAGTG308650.016.2038823
AGTAGCT158850.016.0990665
ACGTCGA17200.016.0681131
GCTGGGA465700.016.0332169
CGAGTAG38350.015.885973
TAGCTGG183300.015.729577
CCGAGTA47100.014.8900572
TACTCGG51300.014.7756663
GCTACTC111300.014.0710281
AGTGCTG339100.013.5790846
CGTCGAG21550.013.477792
GAGTAGC107450.013.317594
GTAGCTG197650.013.2255726
GCTACTT112100.011.9477161
CCCGAGT77100.011.7647921
Produced by FastQC (version 0.11.5)