FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15203106
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC232200.019.9136854
CTACTCG37400.019.880092
CCAAAGT276700.018.0418342
GCTGGGA454500.017.5617549
AAGTGCT266500.017.244635
CAAAGTG289150.016.9712433
CGAGTAG40950.016.9463333
AGTAGCT155400.016.7688485
CCCAAAG347850.016.6559941
CCGAGTA47250.016.6349642
TACTCGG46300.016.5175763
TAGCTGG182050.016.2607527
GCTACTC112350.016.0128971
GAGTAGC106100.015.2834294
AGTGCTG329650.014.3067316
GTAGCTG193900.013.4762126
GGAGGCT345300.013.3319799
TTGGGAG268650.012.9423736
CCCGAGT73300.012.7549641
GTGCTGG420300.012.165587
Produced by FastQC (version 0.11.5)