FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15259774
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC237850.019.8531914
CGAGTAG40650.018.6357963
GCTGGGA458950.018.269979
CCAAAGT289650.018.0908832
AAGTGCT269800.017.8704575
CCCAAAG345900.017.7685951
CTACTCG41800.017.4486792
CAAAGTG292600.017.1310373
AGTAGCT157750.017.0098425
TAGCTGG185050.016.2251367
CCGAGTA48200.015.7194882
GAGTAGC109150.015.17748454
AGTGCTG328800.015.0958626
GCTACTC109800.015.0431271
TACTCGG50650.014.81668953
GTAGCTG195000.014.1612546
GGAGGCT346950.013.2269469
GTGCTGG420400.013.0205217
TGCTGGG484450.012.0156898
CTACTTG111150.011.7222232
Produced by FastQC (version 0.11.5)