FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15405534
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC234700.020.5112174
CTACTCG41750.020.1807292
CCAAAGT287950.018.7363192
CAAAGTG292450.018.0582563
CCCAAAG348650.017.480581
AAGTGCT273650.017.4368135
GCTGGGA467400.017.3610559
CGAGTAG40900.016.9625573
TAGCTGG179200.016.6329527
GCTACTC112350.016.2739751
TACTCGG49650.016.2545223
AGTAGCT153250.016.2147145
CCGAGTA48300.015.5383582
AGTGCTG341150.014.2982726
GAGTAGC106700.013.99953654
GTAGCTG186750.013.988646
GGAGGCT350800.013.7860989
GCTACTT121400.013.7181711
TTGGGAG272650.012.8012136
GTGCTGG429200.012.1076987
Produced by FastQC (version 0.11.5)