FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14727558
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC225650.019.4953424
CGAGTAG37350.019.1560233
CTACTCG38300.019.050922
CCAAAGT279750.018.4348162
AAGTGCT259500.017.6347685
CCGAGTA43800.017.6290912
CCCAAAG344450.017.1797281
GCTGGGA447250.016.9946489
AGTAGCT150750.016.6349125
CAAAGTG292650.016.5328143
TAGCTGG173250.016.2327967
TACTCGG48050.015.9222693
GAGTAGC103000.014.5805274
GCTACTC105500.014.5096611
AGTGCTG326550.014.0789286
GTAGCTG184000.013.7058756
CCCGAGT74050.012.6329531
CCTATAT64050.012.503031
GGAGGCT326050.012.470699
GTGCTGG410500.012.2351467
Produced by FastQC (version 0.11.5)