FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17223276
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC259100.019.6054764
CCAAAGT320900.018.2615782
CGAGTAG39950.017.4035783
CTACTCG40750.017.0618632
CCCAAAG403350.016.7656631
GCTGGGA503550.016.5537649
AAGTGCT308800.016.541965
CAAAGTG328300.016.4669553
CGTCGAG24800.016.023091
AGTAGCT164700.015.2059265
TAGCTGG188450.015.1714847
CCGAGTA47500.014.6372822
GCTACTC121000.014.4851011
TACTCGG51450.014.3409263
AGTGCTG381200.013.9585046
GAGTAGC113350.012.7059254
GTAGCTG205000.012.6665326
GTGCTGG475150.011.7506857
GGAGGCT372200.011.588549
ACGTCGA19750.011.4972191
Produced by FastQC (version 0.11.5)