FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17453904
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC268450.018.944974
CCAAAGT328900.017.6206232
CTACTCG44800.017.4171772
AAGTGCT308750.016.9086555
GCTGGGA518550.016.564829
CCCAAAG405500.016.4461691
CAAAGTG336850.016.3192753
CGAGTAG43350.016.1987613
AGTAGCT172850.015.1834545
TAGCTGG193950.015.0306947
AGTGCTG374650.014.3509836
GCTACTC122400.014.2587511
CGTCGAG22150.014.0931061
CCGAGTA52300.013.8344392
TACTCGG55500.013.8027773
GAGTAGC116100.013.5630994
GTGCTGG484650.012.3374767
GTAGCTG209950.012.2301246
GGAGGCT380100.011.719129
TGCTGGG553750.011.5537658
Produced by FastQC (version 0.11.5)