FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17453904
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC264600.019.9079534
CCAAAGT325550.018.2488422
AAGTGCT302400.017.4913485
CAAAGTG332350.016.9371383
CCCAAAG396600.016.9335061
GCTGGGA513250.016.637849
CTACTCG42800.016.067082
CGAGTAG42250.015.9408723
TAGCTGG185500.015.404627
GCTACTC119450.014.545531
AGTAGCT168800.014.4494815
AGTGCTG369600.014.3309026
TACTCGG54300.014.1006513
CCGAGTA51850.014.0830742
GAGTAGC114700.012.8566434
GTGCTGG470000.012.1296447
GTAGCTG207100.012.0859146
GGAGGCT386850.011.8987559
TGCTGGG543950.011.5772348
TTGGGAG297500.011.0590016
Produced by FastQC (version 0.11.5)