FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17028740
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC266650.019.7273224
CCAAAGT312350.018.428492
CGAGTAG38600.017.8139783
AAGTGCT303500.017.5673315
CCCAAAG392600.017.2942071
GCTGGGA502600.017.1765379
CTACTCG42350.017.0734772
CAAAGTG323700.016.9720953
CCGAGTA47500.016.1177852
AGTAGCT161800.014.9862945
AGTGCTG372950.014.8668216
TACTCGG49950.014.7595913
TAGCTGG188650.014.4369957
GAGTAGC107500.014.178684
GCTACTC116800.013.9101451
GGAGGCT368050.012.79760559
GTGCTGG470350.012.4253447
GTAGCTG205300.011.9494836
CCCGAGT77250.011.9394371
TGCTGGG533100.011.8812748
Produced by FastQC (version 0.11.5)