FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17331874
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG41850.019.6522792
AAAGTGC270700.019.611914
CCAAAGT325350.018.043882
CCCAAAG396650.017.1199911
TACTCGG49700.016.968973
AAGTGCT311600.016.8577545
CAAAGTG338800.016.7762893
GCTGGGA503850.016.4702639
CGAGTAG43300.016.0399513
GCTACTC118300.015.8350071
AGTAGCT162650.015.0799875
TAGCTGG191350.014.5618657
CCGAGTA49750.014.3939082
GGAGGCT378700.013.8221959
AGTGCTG386250.013.8022016
GAGTAGC117700.013.5497844
GTAGCTG208850.011.9143856
GTGCTGG479600.011.8414877
GCTACTT127700.011.72440051
TGCTGGG548050.011.6460348
Produced by FastQC (version 0.11.5)