FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17028740
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC262600.019.0726414
CTACTCG38800.018.8319872
CGTCGAG24800.018.592851
CCAAAGT325650.017.8193932
CCCAAAG394700.016.5889471
AAGTGCT310850.016.5229855
CAAAGTG335850.016.5178413
GCTGGGA497350.016.3603749
TACTCGG48900.015.812833
GCTACTC113450.015.1944891
ACGTCGA21700.015.0377331
AGTAGCT172350.014.5299555
CGAGTAG41200.014.463523
TAGCTGG188200.014.0602797
AGTGCTG383250.013.6053556
GAGTAGC113950.012.8248924
CCGAGTA47500.012.694512
GTCGAGA37450.012.6915212
GGAGGCT370050.012.4224299
GTGCTGG471200.012.0144057
Produced by FastQC (version 0.11.5)