FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14140716
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA15400.020.3748821
AAAGTGC206250.017.7709564
CTACTCG29700.017.2868612
CCAAAGT248350.016.8831422
CCCAAAG310200.015.6555561
CGTCGAG21650.015.4803262
AAGTGCT240400.015.4245895
CAAAGTG257750.015.2161263
GCTGGGA387950.014.7246869
CGAGTAG32100.013.7729433
GCTACTC91550.013.2420031
TACTCGG39000.012.9817693
AGTGCTG306850.012.5028366
AGTAGCT124200.012.0570875
GTCGAGA28750.011.9053872
TAGCTGG143300.011.3966867
CCGAGTA38350.011.1564492
GCTAATT99200.010.8549641
GAGTAGC90300.010.7397164
GGAGGCT300550.010.6541529
Produced by FastQC (version 0.11.5)