FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14504765
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGC201900.018.5517274
CCAAAGT245900.017.410192
CCCAAAG316300.015.8651361
AAGTGCT238150.015.7570185
CAAAGTG258850.015.4627953
CTACTCG36050.015.0424692
GCTGGGA402500.014.9610449
TACTCGG44150.013.5744413
AGTGCTG304600.013.1631636
TAGCTGG146850.012.4378517
CCTATAT60250.012.2030351
GCTACTC91500.012.0920031
AGTAGCT128300.011.5657985
GTGCTGG392050.010.7735687
TGCTGGG456400.010.5522488
GGAGGCT307000.010.4815039
CGAGTAG29450.010.4173323
GAGTAGC86050.010.1151044
GCTAATT98300.09.9484341
CCTATAG58550.09.8751971
Produced by FastQC (version 0.11.5)