FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005471789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005471789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14674452
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG33950.017.859262
AAAGTGC209650.017.4542984
CCAAAGT258750.015.7688492
TACTCGG44450.015.5661013
GCTACTC94000.015.1814681
AAGTGCT245000.014.9656725
CCCAAAG329900.014.8588791
CAAAGTG270400.014.5089473
GCTGGGA411600.014.2111839
CGAGTAG32300.013.6920683
GCTATAT60500.012.8553171
CGTCGAG19350.012.537461
AGTGCTG306400.011.7570626
TAGCTGG146050.011.3801017
CCGAGTA40550.011.2583162
GGAGGCT308650.010.9778959
CCTATAT59150.010.9773681
AGTAGCT136850.010.8943335
CTACTTG96750.010.2482142
TGCTGGG461500.010.1706548
Produced by FastQC (version 0.11.5)